KMID : 1132720170150030098
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Genomics & Informatics 2017 Volume.15 No. 3 p.98 ~ p.107
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Genomic Analysis of miR-21-3p and Expression Pattern with Target Gene in Olive Flounder
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Jo A-Ra
Lee Hee-Eun Kim Heui-Soo
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Abstract
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MicroRNAs (miRNAs) act as regulators of gene expression by binding to the 3¡¯ untranslated region (UTR) of target genes. They perform important biological functions in the various species. Among many miRNAs, miR-21-3p is known to serve vital functions in development and apoptosis in olive flounder. Using genomic and bioinformatic tools, evolutionary conservation of miR-21-3p was examined in various species, and expression pattern was analyzed in olive flounder. Conserved sequences (5¡¯-CAGUCG-3¡¯) in numerous species were detected through the stem-loop structure of miR-21-3p. Thus, we analyzed target genes of miR-21-3p. Among them, 3¡¯ UTR region of PPIL2 gene indicated the highest binding affinity with miR-21-3p based on the minimum free energy value. The PPIL2 gene showed high expression levels in testis tissue of the olive flounder, whereas miR-21-3p showed rather ubiquitous expression patterns except in testis tissue, indicating that miR-21-3p seems to control the PPIL2 gene expression in a complementary repression manner in various tissues of olive flounder. Taken together, this current study contributes to infer the target gene candidates for the miR-21-3p using bioinformatics tools. Furthermore, our data offers important information on the relationship between miR-21-3p and target gene for further functional study.
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KEYWORD
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conserved sequence, gene expression, microRNAs, minimum free energy, 3¡¯untranslated regions
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